Strain Data Sheet

RBRC06239

Strain Information

Image
BRC No.RBRC06239
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Siglec1<tm1(cre)Mtka>
Former Common nameCD169-Cre, B6-Siglec1/Cre KI
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Masato Tanaka, Tokyo University of Pharmacy and Life Sciences, School of Life Science. B6JN/1 ES cells were used. C57BL/6 background.
Strain descriptionCD169 (Siglec1) cre knock-in mice. iCre recombinase is expressed from CD169 locus.
Colony maintenanceHomozygote x Homozygote [or crossing to C57BL/6JJcl]
References
Codon-improved Cre recombinase (iCre) expression in the mouse.
Shimshek D R, Kim J, Hubner M R, Spergel D J, Buchholz F, Casanova E, Stewart A F, Seeburg P H, Sprengel R
Genesis, 32(1):19-26 (2002). 11835670

Vascular-resident CD169-positive monocytes and macrophages control neutrophil accumulation in the kidney with ischemia-reperfusion injury.
Karasawa K, Asano K, Moriyama S, Ushiki M, Monya M, Iida M, Kuboki E, Yagita H, Uchida K, Nitta K, Tanaka M
J. Am. Soc. Nephrol., 26, 896-906 (2015). 25266072

Intestinal CD169(+) macrophages initiate mucosal inflammation by secreting CCL8 that recruits inflammatory monocytes.
Asano K, Takahashi N, Ushiki M, Monya M, Aihara F, Kuboki E, Moriyama S, Iida M, Kitamura H, Qiu C H, Watanabe T, Tanaka M
Nat. Commun., 6:7802 (2015). 26193821

Health Report

Examination Date / Room / Rack2024/12/09Room:3-2Rack:CSentinel mouse program
2024/09/09Room:3-2Rack:CSentinel mouse program
2024/06/10Room:3-2Rack:CSentinel mouse program
2024/03/11Room:3-2Rack:CSentinel mouse program
2023/12/11Room:3-2Rack:CSentinel mouse program
2023/09/11Room:3-2Rack:CSentinel mouse program
2023/06/13Room:3-2Rack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Siglec1
MGI:99668		sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(cre)Mtka>
MGI:1934021		targeted mutation 1, Masato Tanaka
iCre phage P1 codon improved Cre (iCre)2iCre
neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)
polyoma virus polyA2

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 improved Cre recombinase, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyoma virus polyA, mouse Siglec1 genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Am. Soc. Nephrol., 26, 896-906 (2015). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER, and then include them as co-authors in any publications resulted by the use of the BIOLOGICAL RESOURCE.
    DepositorMasato Tanaka (Tokyo University of Pharmacy and Life Sciences)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Camara A, Lavanant AC, Abe J, Desforges HL, Alexandre YO, Girardi E, Igamberdieva Z, Asano K, Tanaka M, Hehlgans T, Pfeffer K, Pfeffer S, Mueller SN, Stein JV, Mueller CG.
    CD169+ macrophages in lymph node and spleen critically depend on dual RANK and LTbetaR signaling.
    Proc Natl Acad Sci U S A 119(3) (2022) 35031565
    Millard SM, Heng O, Opperman KS, Sehgal A, Irvine KM, Kaur S, Sandrock CJ, Wu AC, Magor GW, Batoon L, Perkins AC, Noll JE, Zannettino ACW, Sester DP, Levesque JP, Hume DA, Raggatt LJ, Summers KM, Pettit AR.
    Fragmentation of tissue-resident macrophages during isolation confounds analysis of single-cell preparations from mouse hematopoietic tissues.
    Cell Rep 37(8) 110058(2021) 34818538
    Camara A, Cordeiro OG, Alloush F, Sponsel J, Chypre M, Onder L, Asano K, Tanaka M, Yagita H, Ludewig B, Flacher V, Mueller CG.
    Lymph Node Mesenchymal and Endothelial Stromal Cells Cooperate via the RANK-RANKL Cytokine Axis to Shape the Sinusoidal Macrophage Niche.
    Immunity 50(6) 1467-1481.e6(2019) 31201093