Strain Data Sheet

RBRC06257

Strain Information

Image
BRC No.RBRC06257
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(H4K20me1-mCherry)Osb>/44
Former Common nameConditional H4K20me1-mCherry#44, B6-ROSA26/Neo/mintbody KI#44
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Kimura, Yuko Sato, Jun Ueda and Kazuo Yamagata, Osaka University in 2012. EGR-G101 ES cells derived from C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb were used.
Strain descriptionROSA26 H4K20me1-mCherry conditional knock-in mice (FLP-frt system).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References
Genetically encoded system to track histone modification in vivo.
Yuko Sato, Masanori Mukai, Jun Ueda, Michiko Muraki, Timothy J Stasevich, Naoki Horikoshi, Tomoya Kujirai, Hiroaki Kita, Taisuke Kimura, Seiji Hira, Yasushi Okada, Yoko Hayashi-Takanaka, Chikashi Obuse, Hitoshi Kurumizaka, Atsuo Kawahara, Kazuo Yamagata, Naohito Nozaki, Hiroshi Kimura
Sci. Rep., 3, 2436 (2013). 23942372

Visualizing histone H4K20me1 in knock-in mice expressing the mCherry-tagged modification-specific intracellular antibody.
Yuko Sato, Maoko Takenoshita, Miku Ueoka, Jun Ueda, Kazuo Yamagata, Hiroshi Kimura
Histochem. Cell Biol., 162(1-2):41-52 (2024). 38762823

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site6Frt
Frt yeast FRT (flippase recombination target) site6Frt
Gt(ROSA26)Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA26)Sor
SA Splice acceptor (adenovirus)6SA
mCherry Red Fluorescent Protein (Discosoma sp.)6mCherry
neo neomycin resistance gene (E. coli)6
mouse I5F11 antibody variable domain6
SV40 polyA signal6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse I5F11 antibody variable domain cDNA, E. coli Neomycine resistance gene, yeast FRT (flipase recombination target) sites, Discosoma sp. mCherry cDNA, adenovirus splice acceptor, SV40 polyadenylation sequence
    Research applicationCell Biology Research
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Histochem. Cell Biol., 162(1-2):41-52 (2024).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Tokyo lnstitute of Technology (https://www.titech.ac.jp/).
    DepositorHiroshi Kimura (Tokyo Institute of Technology)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: FP license of TBUSA
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data