Strain Data Sheet

RBRC06258

Strain Information

Image
BRC No.RBRC06258
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Cacng5-EGFP)500Bsi
Former Common nameCacng5 promoter-GFP, B6-Cacng5-GFP Tg#500
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshiaki Shinohara and Hajime Hirose, RIKEN Brain Science Institute. The BAC transgenic construct containing the mouse Cacng5 gene with IRES-GFP (GENSAT1-BX1300) was used. C57BL/6 background.
Strain descriptionhese transgenic mice express IRES-GFP under the control of the mouse Cacng5 promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
References
Hippocampal CA3 and CA2 have distinct bilateral innervation patterns to CA1 in rodents.
Shinohara Y, Hosoya A, Yahagi K, Ferecsko A S, Yaguchi K, Sik A, Itakura M, Takahashi M, Hirase H
Eur. J. Neurosci., 35, 702-710 (2012) 22339771

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP mouse Cacng5 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse BAC clone (RP23-329L1) containing mouse Cacng5 genomic DNA, jellyfish enhanced green fluorescent protein cDNA, bovine growth hormone polyadenylation signal, E. coli ampicilline resistance gene, E. coli R6Kgamma origin of replication
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. J. Neurosci., 35, 702-710 (2012). The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a mention of the GENSAT Project (GENSAT1-BX1300) as the source of the original BAC transgenic construct are requested.
    DepositorHajime Hirase (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data