Strain Data Sheet

RBRC06270

Strain Information

Image
BRC No.RBRC06270
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tgm3<tm1a(KOMP)Wtsi>/B12
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)2En2
Frt yeast FRT (flippase recombination target) site2Frt
Frt yeast FRT (flippase recombination target) site2Frt
IRES internal ribosomal entry site (EMCV)2
Tgm3
MGI:98732		transglutaminase 3, E polypeptide2Tgm3<tm1a(KOMP)Wtsi>
MGI:5294973		targeted mutation 1a, Wellcome Trust Sanger Institute
  • uncombable hair syndrome 2(MedGEN)
    • lacZ beta-galactosidase (E. coli)2
    loxP phage P1 loxP2loxP
    loxP phage P1 loxP2loxP
    loxP phage P1 loxP2loxP
    neo neomycin resistance gene (E. coli)2
    SV40 polyA signal2

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast Frt sites, En2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, Bacteriophage P1 loxP sites, human beta actin promoter, E. coli Neomycin resistance gene, mouse Tgm3 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT should acknowledge the KOMP, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data