Strain Data Sheet

RBRC06289

Strain Information

Image
BRC No.RBRC06289
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tyms<tm1a(EUCOMM)Hmgu>/D03
Former Common name
H-2 Haplotype
ES Cell lineJM8.N4 [C57BL/6N]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)5En2
Frt yeast FRT (flippase recombination target) site5Frt
Frt yeast FRT (flippase recombination target) site5Frt
Ires internal ribosomal entry site (EMCV)5
Tyms
MGI:98878		thymidylate synthase5Tyms<tm1a(EUCOMM)Hmgu>
MGI:54368967		targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
lacZ beta-galactosidase (E. coli)5
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP
neo neomycin resistance gene (E. coli)5 hBactP (human beta actin promoter)
SV40 polyadenylation site5

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEn2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, Bacteriophage P1 loxP site, yeast Frt sites, human beta actin promoter, E. coli Neomycin resistance gene
    Research application
    Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data