Strain Data Sheet

RBRC06291

Strain Information

Image
BRC No.RBRC06291
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ube3c<tm1b(EUCOMM)Hmgu>/B01
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site5Frt
Ires internal ribosomal entry site (EMCV)5
Ube3c
MGI:2140998		ubiquitin protein ligase E3C5Ube3c<tm1b(EUCOMM)Hmgu> targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
lacZ beta-galactosidase (E. coli)5
loxP phage P1 loxP5loxP
SV40 polyA signal5

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal gait(MP:0001406)

  • decreased body length(MP:0001258)

  • decreased bone mineral density(MP:0000063)

  • decreased fluid intake(MP:0011942)

  • decreased food intake(MP:0011940)
    • more 4 phenotypes
  • decreased heart weight(MP:0002834)

  • decreased lean body mass(MP:0003961)

  • decreased respiratory quotient(MP:0010379)

  • increased circulating alkaline phosphatase level(MP:0002968)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) site, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP site, mouse Ube3c genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data