Strain Data Sheet

RBRC06293

Strain Information

Image
BRC No.RBRC06293
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ngly1<tm1.1Tasuz>
Former Common namePNGase KO, B6-Ngly1 KO
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Tadashi Suzuki, Global Research Cluster, RIKEN. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Exons 11 and 12 of Ngly gene were removed by crossing with Cre mice (B6). The mutant mice were backcrosses to C57BL/6 over 13 times.
Strain descriptionNgly gene knockout mice. Exons 11 and 12 were removed. Homozygous mutant mice are lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crj)]
References
Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells.
Huang C, Harada Y, Hosomi A, Masahara-Negishi Y, Seino J, Fujihira H, Funakoshi Y, Suzuki T, Dohmae N, Suzuki T
Proc. Natl. Acad. Sci. USA, 112(5) 1398-403 (2015). 25605922

Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene.
Fujihira H, Masahara-Negishi Y, Tamura M, Huang C, Harada Y, Wakana S, Takakura D, Kawasaki N, Taniguchi N, Kondoh G, Yamashita T, Funakoshi Y, Suzuki T
PLoS Genet., 13(4):e1006696 (2017). 28426790

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site14Frt
Ngly1
MGI:1913276		N-glycanase 114Ngly1<tm1.1Tasuz>
MGI:5705596		targeted mutation 1.1, Tadashi Suzuki
  • obsolete NGLY1-deficiency(MedGEN)
    • loxP phage P1 loxP14loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cell physiology(MP:0005621)

  • embryonic lethality, complete penetrance(MP:0011092)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, yeast FRT (flipase recombination target) site, mouse Ngly1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Mouse Models for Human Disease
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 13(4):e1006696 (2017).
    DepositorTadashi Suzuki (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data