Strain Data Sheet

RBRC06296

Strain Information

Image
BRC No.RBRC06296
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameCAJcl.Cg-Aicda<tm2.1Hon>
Former Common nameBALBcAJcl-AID G23S, BALBcA-AID/G23S KI
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Reiko Shinkura, Nagahama Institute of Bio-Science and Technology, and Tasuku Honjo, Graduate School of Medicine, Kyoto University. Bruce 4 ES cells were used. The mutant mice were backcrossed to BALB/cAJcl.
Strain descriptionAicda G23S mutant knock-in mice. BALB/cAJcl background. For the details, please contact the developer (Dr. Reiko Shinkura, Nagahama Institute of Bio-Science and Technology).
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAJcl]
References
Separate domains of AID are required for somatic hypermutation and class-switch recombination.
Shinkura R, Ito S, Begum N A, Nagaoka H, Muramatsu M, Kinoshita K, Sakakibara Y, Hijikata H, Honjo T
Nat. Immunol., 5, 707-712 (2004). 15195091

Mice carrying a knock-in mutation of Aicda resulting in a defect in somatic hypermutation have impaired gut homeostasis and compromised mucosal defense.
Wei M, Shinkura R, Doi Y, Maruya M, Fagarasan S, Honjo T
Nat. Immunol., 12, 264-270 (2011). 21258321

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aicda
MGI:1342279		activation-induced cytidine deaminase6Aicda<tm2.1Hon>
MGI:4942397		targeted mutation 2.1, Tasuku Honjo
  • hyper-IgM syndrome type 2(MedGEN)
    • loxP phage P1 loxP6loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal gut flora balance(MP:0010377)

  • decreased IgG1 level(MP:0008495)

  • immune system phenotype(MP:0005387)

  • increased germinal center B cell number(MP:0008177)

  • increased susceptibility to bacterial infection induced morbidity/mortality(MP:0009788)
    • more 1 phenotypes
  • increased susceptibility to bacterial infection(MP:0002412)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Aicda (G23S) genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Immunol., 12, 264-270 (2011).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data