Strain Data Sheet

RBRC06299

Strain Information

Image
BRC No.RBRC06299
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameCAnPt.Cg-Aicda<tm1Hon>
Former Common nameBALBcAnPt Aicda KO, BALBPt-AID KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Reiko Shinkura, Nagahama Institute of Bio-Science and Technology, and Tasuku Honjo, Graduate School of Medicine, Kyoto University. TT2 ES cells were used. The mutant mice were backcrossed to BALB/cAJcl over 5 times.
Strain descriptionAicda knockout mice. BALB/cAnPt background. For the details, please contact the developer (Dr. Reiko Shinkura, Nagahama Institute of Bio-Science and Technology).
Colony maintenanceHomozygote x Homozygote
References
Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Muramatsu M, Kinoshita K, Fagarasan S, Yamada S, Shinkai Y, Honjo T
Cell, 102, 553-563 (2000). 11007474

Critical roles of activation-induced cytidine deaminase in the homeostasis of gut flora.
Fagarasan S, Muramatsu M, Suzuki K, Nagaoka H, Hiai H, Honjo T
Science, 298, 1424-1427 (2002). 12434060

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aicda
MGI:1342279		activation-induced cytidine deaminase6Aicda<tm1Hon>
MGI:2156156		targeted mutation 1, Tasuku Honjo
  • hyper-IgM syndrome type 2(MedGEN)
    • loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal class switch recombination(MP:0004816)

  • abnormal somatic hypermutation frequency(MP:0004815)

  • decreased IgA level(MP:0001807)

  • decreased IgG level(MP:0001805)

  • decreased IgG1 level(MP:0008495)
    • more 6 phenotypes
  • decreased IgG2a level(MP:0008496)

  • decreased IgG2b level(MP:0008497)

  • decreased IgG3 level(MP:0008498)

  • increased IgM level(MP:0002494)

  • increased germinal center B cell number(MP:0008177)

  • increased susceptibility to bacterial infection induced morbidity/mortality(MP:0009788)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Aicda genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science, 298, 1424-1427 (2002).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data