Strain Data Sheet

RBRC06303

Strain Information

Image
BRC No.RBRC06303
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Malat1<tm1.1Shna>/ShnaRbrc
Former Common nameMalat1 KO, B6-Malat1 KO, NEAT2(lacZ)(Acc.No.CDB0837K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichi Nakagawa, RIKEN Advanced Science Institute. TT2 ES cells were used to generate the knockout mice. A FRT-flanked neo cassette was removed by crossing with FLP mice. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionMalat1 ncRNA knock-out mice. Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References
Malat1 is not an essential component of nuclear speckles in mice.
Nakagawa S, Ip J Y, Shioi G, Tripathi V, Zong X, Hirose T, Prasanth K V
RNA, 18, 1487-1499 (2012). 22718948

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site19Frt
Malat1
MGI:1919539		metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA)19Malat1<tm1.1Shna> targeted mutation 1.1, Shinichi Nakagawa
lacZ beta-galactosidase (E. coli)19
lox71 mutant lox sequence19lox71
loxP phage P1 loxP19loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 lox71 site, E. coli lacZ, polyA, yeast FRT (flipase recombination target) site, phage P1 loxP site, polyA, mouse Malat1 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No.CDB0837K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (RNA. 2012 18(8):1487-99.). (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ben-Crentsil NA, Mohammed Ismail W, Balasis ME, Newman H, Quintana A, Binder M, Kruer T, Neupane S, Ferrall-Fairbanks MC, Fernandez J, Lasho TL, Finke CM, Ibrahim ML, McGraw KL, Wysota M, Aldrich AL, Ryder CB, Letson CT, Traina J, McLemore AF, Droin N, Shastri A, Yun S, Solary E, Sallman DA, Beg AA, Ma L, Gaspar-Maia A, Patnaik MM, Padron E.
    RNA shielding of P65 is required to potentiate oncogenic inflammation in TET2 mutated clonal hematopoiesis.
    Cancer Discov (2024) 39189614