Strain Data Sheet

RBRC06355

Strain Information

Image
BRC No.RBRC06355
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Nes-EGFP)1Yamm
Former Common nameNestin-EGFP, B6-Nes-GFP Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masahiro Yamaguchi, PRESTO, Japan Science and Technology Corporation in 1998. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. C57BL/6 and DBA/2 mixed background.
Strain descriptionNestin-GFP reporter mice, expressing a EFGP gene under the control of rat nestin promoter.
Colony maintenanceCarrier x Carrier
References
Visualization of neurogenesis in the central nervous system using nestin promoter-GFP transgenic mice.
Yamaguchi M, Saito H, Suzuki M, Mori K
NeuroReport, 11, 1991-1996 (2000). 10884058

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP rat Nestin promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNARat nestin genomic DNA, jellyfish EGFP cDNA, rabbit beta globin polyA
    Research applicationFluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. NeuroReport, 11, 1991-1996 (2000).
    DepositorMasahiro Yamaguchi (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Daido W, Nakashima T, Masuda T, Sakamoto S, Yamaguchi K, Horimasu Y, Miyamoto S, Iwamoto H, Fujitaka K, Hamada H, Hattori N.
    Nestin and Notch3 collaboratively regulate angiogenesis, collagen production, and endothelial-mesenchymal transition in lung endothelial cells.
    Cell Commun Signal 21(1) 247(2023) 37735673
    Sawada R, Nakano-Doi A, Matsuyama T, Nakagomi N, Nakagomi T.
    CD44 expression in stem cells and niche microglia/macrophages following ischemic stroke.
    Stem Cell Investig 7 4(2020) 32309418