Strain Data Sheet

RBRC06390

Strain Information

Image
BRC No.RBRC06390
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Fbxl5<tm2.1Kei>
Former Common nameFbxl5 cKO, B6-Fbxl5 floxed
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J (N7 generations).
Strain descriptionFbx15 gene floxed mice (Flox type). Exons 4 and 5 were flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJcl]
References
The FBXL5-IRP2 axis is integral to control of iron metabolism in vivo.
Moroishi T, Nishiyama M, Takeda Y, Iwai K, Nakayama K I
Cell Metabolism, 14, 339-351 (2011). 21907140

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fbxl5
MGI:2152883		F-box and leucine-rich repeat protein 55Fbxl5<tm2.1Kei>
MGI:5295289		targeted mutation 2.1, Keiichi I Nakayama
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal blood coagulation(MP:0002551)

  • abnormal circulating enzyme level(MP:0001570)

  • abnormal hepatocyte morphology(MP:0000607)

  • abnormal iron level(MP:0001770)

  • abnormal liver physiology(MP:0000609)
    • more 15 phenotypes
  • abnormal mitochondrial physiology(MP:0006036)

  • decreased circulating unsaturated transferrin level(MP:0011897)

  • hepatic steatosis(MP:0002628)

  • increased circulating alanine transaminase level(MP:0002941)

  • increased circulating aspartate transaminase level(MP:0005343)

  • increased circulating iron level(MP:0008810)

  • increased lactate dehydrogenase level(MP:0002944)

  • increased liver iron level(MP:0008807)

  • increased partial thromboplastin time(MP:0012359)

  • increased prothrombin time(MP:0012356)

  • liver failure(MP:0003326)

  • liver hemorrhage(MP:0003888)

  • liver inflammation(MP:0001860)

  • oxidative stress(MP:0003674)

  • pale liver(MP:0000603)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, mouse Fbxl5 genomic DNA
    Research applicationCre/loxP system
    Hematological Research
    Metabolism Research
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorKeiichi Nakayama (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data