Strain Data Sheet

RBRC06394

Strain Information

Image
BRC No.RBRC06394
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Fbxw7<tm1Kin>
Former Common nameFbxw7 KO, B6-Fbxw7 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J over 7 times.
Strain descriptionFbxw7 gene knockout mice. Exons 1 to 5 was replaced with PGK-neo (loxP-neo) cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Mouse Fbw7/Sel-10/Cdc4 is required for notch degradation during vascular development.
Tsunematsu R, Nakayama K, Oike Y, Nishiyama M, Ishida N, Hatakeyama S, Bessho Y, Kageyama R, Suda T, Nakayama K I
J. Biol. Chem., 279, 9417-9423 (2004). 14672936

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fbxw7
MGI:1354695		F-box and WD-40 domain protein 73Fbxw7<tm1Kin>
MGI:3036571		targeted mutation 1, Kei-ichi Nakayama
loxP phage P1 loxP3loxP
loxP phage P1 loxP3loxP
neo neomycin resistance gene (E. coli)3 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal3

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal brain vasculature morphology(MP:0004950)

  • abnormal neural tube closure(MP:0003720)

  • abnormal somite border morphology(MP:0012728)

  • abnormal somite development(MP:0001688)

  • abnormal vascular development(MP:0000259)
    • more 8 phenotypes
  • abnormal vasculogenesis(MP:0001622)

  • abnormal vitelline vascular remodeling(MP:0004076)

  • decreased angiogenesis(MP:0005602)

  • embryonic growth retardation(MP:0003984)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • intracranial hemorrhage(MP:0001915)

  • pallor(MP:0003717)

  • pericardial edema(MP:0001787)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, mouse phosphoglycerate kinase promoter, SV40 polyA signal, phage P1 loxP
    Research applicationHematological Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorKeiichi Nakayama (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Mouse of the Month Jan 2015

    BRC mice in Publications

    No Data