Strain Data Sheet

RBRC06412

Strain Information

Image
BRC No.RBRC06412
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6;129-Wtap<Gt(OST200529)Lex>
Former Common nameWtap KO, Wtap Gt
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takao Hamakubo, Research Center for Advanced Science and Technology, University of Tokyo. C57BL/6 and 129 mixed background.
Strain descriptionWtap gene gene-trap mice. Homozygous mutant mice are embryonic lethal (around E6.5).
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6J(Crlj)]
References
Wilms' tumor 1-associating protein regulates G2/M transition through stabilization of cyclin A2 mRNA.
Horiuchi K, Umetani M, Minami T, Okayama H, Takada S, Yamamoto M, Aburatani H, Reid P C, Housman D E, Hamakubo T, Kodama T
Proc. Natl. Acad. Sci. USA, 103, 17278-17283 (2006). 17088532

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
BTK Burton's tyrosine kinase17BTK
LTR retrovirus LTR (viral long terminal repeat)17LTR
Wtap
MGI:1926395		WT1 associating protein17Wtap<Gt(OST200529)Lex>
MGI:35331203		gene trap OST178967, Lexicon Genetics
lacZ beta-galactosidase (E. coli)17
neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal17

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal embryo development(MP:0001672)

  • embryonic lethality, complete penetrance(MP:0011092)
    • Detailed phenotype data

    Ordering Information

    Donor DNAretrovirus　LTR, マウス PGK promoter, ヒト BTK exon, simian virusSV40 polyA, 放線菌 Neomycin, 大腸菌LacZ
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 103, 17278-17283 (2006).
    DepositorTakao Hamakubo (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data