Strain Data Sheet

RBRC06427

Strain Information

Image
BRC No.RBRC06427
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm1(CAG-SCAT1)Miur>
Former Common nameRosa26-CAG-STOP-SCAT1, B6-ROSA26/SCAT1floxed KI
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Ting Liu, Yoshifumi Yamaguchi and Masayuki Miura, Graduate School of Pharmaceutical Sciences, University of Tokyo, and Katsuaki Hoshino and Tsuneyasu Kaisho, RIKEN Research Center for Allergy and Immunology. C57BL/6J and C57BL/6N mixed.
Strain descriptionCAG-STOP-CAST1 knock-in mice. A CAG-loxP-neo-loxP-SCAT1-pA cassette was inserted into the Gt(ROSA)26Sor locus. Homozygous mice are viable (fertility is unknown).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response.
Liu T, Yamaguchi Y, Shirasaki Y, Shikada K, Yamagishi M, Hoshino K, Kaisho T, Takemoto K, Suzuki T, Kuranaga E, Ohara O, Miura M
Cell Rep., 8(4):974-982 (2014). 25127135

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ECFP Enhanced Cyan Fluorescent Protein (Aequorea victoria)6ECFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
Frt yeast FRT (flippase recombination target) site6Frt
GH Growth hormone polyA (Bovine)6GH
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-SCAT1)Miur> targeted mutation 1, Masayuki Miura
Venus Yellow Fluorescent Protein (Aequorea victoria)6Venus
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
SV40 polyA signal6

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer,chicken beta-actin promoter, rabbit beta-globin genomic DNA), SCAT1 (Jellyfish ECFP cDNA, caspase-1 cleavage site, Jellyfish Venus cDNA), herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin resistance gene, phage P1 loxP sites, Bovine polyA, SV40 polyA, yeast FRT (flipase recombination target) site, mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 8(4):974-982 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorMasayuki Miura (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)

    BRC mice in Publications

    No Data