Strain Data Sheet

RBRC06442

Strain Information

Image
BRC No.RBRC06442
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Atp13a2<tm1b(EUCOMM)Hmgu>/F08
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NTac
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Atp13a2
MGI:1922022		ATPase type 13A24Atp13a2<tm1b(EUCOMM)Hmgu>
MGI:5587050		targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
  • autosomal recessive spastic paraplegia type 78(MedGEN)

  • Kufor-Rakeb syndrome(MedGEN)
    • En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)4En2
    Frt yeast FRT (flippase recombination target) site4Frt
    Frt yeast FRT (flippase recombination target) site4Frt
    Ires internal ribosomal entry site (EMCV)4
    lacZ beta-galactosidase (E. coli)4
    loxP phage P1 loxP4loxP
    SV40 polyA4

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased memory-marker CD4-positive NK T cell number(MP:0013522)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEn2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, Bacteriophage P1 loxP site, yeast Frt sites
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data