Strain Data Sheet

RBRC06463

Strain Information

Image
BRC No.RBRC06463
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Prkcd<tm1.1Shb>
Former Common namePKCdelta KO, B6-Prkcd KO
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Yuko Niino and Seiji Shiota, School of Medicine, Showa University and Tomoya Nakamachi, Graduate School of Science and Engineering for Education, University of Toyama. The mutant mice were generated from Prkcd<tm1Shb> mice (RBRC06462) by crossing with a Tg(Pgk2-cre)24Shb mice (PGK2-Cre transgenic mice).
Strain descriptionPrkcd gene knockout mice. Exon 7 of the Prkcd was deleted. Homozygous mutant mice are probably embryonic lethal. Under investigation.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References
PKCdelta deficiency inhibits fetal development and is associated with heart elastic fiber hyperplasia and lung inflammation in adult PKCdelta knockout mice.
Niino Y S, Kawashima I, Iguchi Y, Kanda H, Ogura K, Mita-Yoshida K, Ono T, Yamazaki M, Sakimura K, Yogosawa S, Yoshida K, Shioda S, Gotoh T
PLoS One, 16(7):e0253912 (2021). 34197550

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Prkcd
MGI:97598		protein kinase C, delta14Prkcd<tm1.1Shb> targeted mutation 1.1, Seiji Shiota
  • autoimmune lymphoproliferative syndrome, type III caused by mutation in PRKCD(MedGEN)
    • loxP phage P1 loxP14loxP

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • obsolete no phenotypic analysis (MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPharge P1 LoxP site, mouse PKCdelta genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 16(7):e0253912 (2021). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR/DEVELOPER (Drs. Seiji Shiota and Yuko Niino) is requested (will be announced). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR/DEVELOPER and its colleagues (Drs. Tomoya Nakamachi and Jun Watanabe) is requested. Before the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR/DEVELOPER, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER, and then include them as co-authors in the publication.
    DepositorSeiji Shiota (Showa University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data