Strain Data Sheet

RBRC06494

Strain Information

Image
BRC No.RBRC06494
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Grm6<tm1Nak>
Former Common name
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Shigetada Nakanishi, Kyoto University Faculty of Medicine. CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c> were used to generate the mutant mice. C57BL/6 semicongenic.
Strain descriptionGrm6 gene knock-out mice. mGluR6 (glutamate receptor, metabotropic 6) gene is suggested to be responsible for ON responses in both the rod and cone systems. Homozygous mutant mice are viable and fertile. This strain is useful for investigating the promoter function of the cell-specific and developmentally regulated expression of mGluR6.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Masu M, Iwakabe H, Tagawa Y, Miyoshi T, Yamashita M, Fukuda Y, Sasaki H, Hiroi K, Nakamura Y, Shigemoto R, et a l
Cell, 80, 757-765 (1995). 7889569

The mGluR6 5' upstream transgene sequence directs a cell-specific and developmentally regulated expression in retinal rod and ON-type cone bipolar cells.
Ueda Y, Iwakabe H, Masu M, Suzuki M, Nakanishi S
J. Neurosci., 17, 3014-3023 (1997). 9096137

Immunohistological studies of metabotropic glutamate receptor subtype 6-deficient mice show no abnormality of retinal cell organization and ganglion cell maturation.
Tagawa Y, Sawai H, Ueda Y, Tauchi M, Nakanishi S
J. Neurosci., 19, 2568-2579 (1999). 10087070

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grm6
MGI:1351343		glutamate receptor, metabotropic 611Grm6<tm1Nak>
MGI:1857985		targeted mutation 1, Shigetada Nakanishi
  • congenital stationary night blindness 1B(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal eye electrophysiology(MP:0005551)

  • abnormal synaptic transmission(MP:0003635)

  • behavior/neurological phenotype(MP:0005386)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse grm6 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 19, 2568-2579 (1999). J. Neurosci., 17, 3014-3023 (1997). Cell, 80, 757-765 (1995).
    DepositorShigetada Nakanishi (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data