Strain Data Sheet

RBRC06517

Strain Information

Image
BRC No.RBRC06517
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm10(PITT)Maoh>
Former Common nameTOKMO-3, B6-ROSA26/PITT KI
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentDeveloped by Masato Ohtsuka, Tokai University School of Medicine in 2014. EGR101 ES cells were used to generate the mutant mice.
Strain descriptionSA-F14-attP-JT15-eGFP-T2A-neo-hOCT4 ZFN target site-pA-FRT-L-lox2272-F15 ROSA26 knock-in mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6N]
References
One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy C B
BMC Genomics 2015, 16:274 25887549

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP
F14 yeast FRT (flippase recombination target) site (synthetic) variant (F14)6F14
F15 yeast FRT (flippase recombination target) site (synthetic) variant (F15)6F15
FRT-L yeast FRT (flippase recombination target) site, variant (FRT-L)6FRT-L
Frt yeast FRT (flippase recombination target) site6Frt
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm10(PITT)Maoh> targeted mutation 10, Masato Ohtsuka
JT15 phage P1 loxP variant (JT15)6JT15
SA Splice acceptor (adenovirus)6SA
T2A Thosea asigna virus 2A peptide (insect)6T2A
att attachment site6att
att attachment site6att
lox2272 phage P1 loxP variant6lox2272
neo neomycin resistance gene (E. coli)6
SV40 polyA signal6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Gt(ROSA)26Sor, adenovirus SA, yeast FRT variant (F14), Streptomyces lividans and actinophage phi C31 attP, phage P1 loxP variant (JT15), jellyfish eGFP cDNA, insect Thosea asigna virus T2A peptide, E. coli neo, human OCT4 cDNA, Simian virus 40 (SV40) polyA, yeast FRT variant (FRT-L), phage P1 loxP variant (lox2272), yeast FRT variant (F15)
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in the first publication of the results obtained from the use of the BIOLOGICAL RESOURCE.
    DepositorMasato　Ohtsuka (Tokai University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Sep 2015

    BRC mice in Publications

    No Data