Strain Data Sheet

RBRC06574

Strain Information

Image
BRC No.RBRC06574
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Chx10-cre/ERT2)G7Tfur
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2010. The BAC-Chx10-creERT2 transgene was injected into the pronuclei of B6C3F1 fertilized eggs.
Strain descriptionBAC-Chx10-creERT2 transgenic mice. This transgenic mouse expresses tamoxifen-inducible cre recombinase under the control of the mouse Chx10 promoter. 129 background (RBRC05565), C57BL/6 mixed background (RBRC06574).
Colony maintenanceCarrier x Carrier, Noncarrier [or Crossing to C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2
cre Phage P1 Cre recombinaseUNcre mouse Vsx2 promoter
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Vsx2 genomic DNA, P1 phage Cre recombinase, Human Mutated Estrogen receptor LBD, SV40 polyA signal, Phage P1 loxP derived from pBAC vector
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
    DepositorTakahisa Furukawa (Osaka Bioscience Institute)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data