Strain Data Sheet

RBRC06579

Strain Information

Image
BRC No.RBRC06579
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Thy1-G-CaMP7,-DsRed2)492Bsi
Former Common nameThy1-G-CaMP7 Tg, B6-Thy1-GCMP7/DsRed, Tg#492
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masamichi Ohkura and Junichi Nakai, Saitama University, Masaaki Sato and Yasunori Hayashi, RIKEN Brain Institute in 2011. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionThy1-G-CaMP7/DsRed2 transgenic mice. These transgenic mice express G-CAMP7 and DsRed2 under the control of the Thy1 promoter.
Colony maintenanceCarrier x Noncarrier [or Crossing to C57BL/6JJmsSlc]
References
Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals.
Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J
PLoS One, 7, e51286, (2012). 23240011

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
DsRed2 Red Fluorescent Protein (Discosoma sp.)UNDsRed2
GCaMP7 RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP7 mouse Thy1.2 promoter
T2A Thosea asigna virus 2A peptide (insect)UNT2A
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAGCaMP7(RSET tag, Chicken myosin light chain kinase (M13) cDNA, Aequorea Victoria GFP cDNA, Rat calmodulin cDNA), Thosea asigna virus 2A peptide (T2A), Discosoma sp. DsRed2 cDNA, mouse Thy1.2 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 7, e51286, (2012). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorYasunori Hayashi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: TBUSAMTA Nonprofit
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data