Strain Data Sheet

RBRC06802

Strain Information

Image
BRC No.RBRC06802
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Smc2<tm1.1Hiran>
Former Common nameSmc2 floxed, B6-Smc2 floxed
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Kenji Nishide and Tatsuya Hirano, Chromosome Dynamics Laboratory, RIKEN. BA1 ES cells (C57BL/6 x 129/SvEv) were targeted by using standard conditional knockout methods by inGenious Targeting Laboratory (NY, USA). The mice were mated with CAG-FLPe transgenic mice to delete the Neo selection cassettes, and subsequently backcrossed to C57BL/6J over eight generations.
Strain descriptionSmc2 gene floxed mice. Condensins I and II are evolutionarily conserved protein complexes and thought to be central to chromosome assembly and segregation among eukaryotes. In Smc2 floxed mice, exons 10-12 of the Smc2 gene (encoding a shared subunit of both condensins) are flanked by loxP sites. Tissue-specific deletion of Smc2 can be generated by crossing with various Cre-expressing mice to study the functions of both condensins. Homozygous floxed mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
References
Overlapping and non-overlapping functions of condensins I and II in neural stem cell divisions.
Nishide K, Hirano T
PLoS Genet., 10(12):e1004847 (2014). 25474630

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site4Frt
Smc2
MGI:106067		structural maintenance of chromosomes 24Smc2<tm1.1Hiran> targeted mutation 1.1, Tatsuya Hirano
loxP phage P1 loxP4loxP
loxP phage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Smc2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 10(12):e1004847 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorTatsuya Hirano (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data