Strain Data Sheet

RBRC06805

Strain Information

Image
BRC No.RBRC06805
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1(dach1)Hhsk>
Former Common nameDach Tg, B6-ROSA26/Dach1 KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Haruko Hayasaka, Graduate School of Medicine, Osaka University. RENKA ES cells were used to generate the mutant mice.
Strain descriptionDach1 ROSA26 conditional knock-in mice. Targeted insertion of SA-loxP-CAT-PGK-neo-loxP-Dach1-pA cassette into the mouse Rosa26 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dach1 dachshund 1 (Drosophila)6Dach1<tm1Hhsk> targeted mutation 1, Haruko Hayasaka
Frt yeast FRT (flippase recombination target) site6Frt
GH Growth hormone polyA (Bovine)6GH
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(dach1)Hhsk> targeted mutation 1, Haruko Hayasaka
SA Splice acceptor (adenovirus)6SA
cat E. coli chloramphenicol acetyltransferase (CAT)6
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal6

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, E. coli cat, bovine growth hormone polyA signal, mouse Dachshund1 cDNA, adenovirus type2 Splicing acceptor (SA), yeast FRT (flipase recombination target) site, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 polyA signal, mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCancer Research
    Cre/loxP system
    Developmental Biology Research
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT, which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not -for-profit research, is required to enter into a Material Transfer Agreement with Osaka University.
    DepositorHaruko Hayasaka (Kindai University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data