Strain Data Sheet

RBRC06806

Strain Information

Image
BRC No.RBRC06806
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(hOX-EGFP,-cre)1Ahky
Former Common nameOrexin-EGFP-2A-Cre, B6-hOX-GFP/Cre Tg, C57BL/6-Tg(hOX-EGFP,-cre)1Ahky
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Akihiro Yamanaka, Research Institute of Environmental Medicine Nagoya University in 2014. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionOrexin-EGFP-2A-Cre transgenic mice. Tg mice show no obvious abnormality.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Concurrent and robust regulation of feeding behaviors and metabolism by orexin neurons.
Inutsuka A, Inui A, Tabuchi S, Tsunematsu T, Lazarus M, Yamanaka A
Neuropharmacology, 85, 451-460 (2014). 24951857

Health Report

Examination Date / Room / Rack2024/11/18Room:4-ARack:CSentinel mouse program
2024/08/19Room:4-ARack:CSentinel mouse program
2024/05/20Room:4-ARack:CSentinel mouse program
2024/02/19Room:4-ARack:CSentinel mouse program
2023/11/20Room:4-ARack:CSentinel mouse program
2023/11/13Room:4-ARack:CAdditional tests
2023/08/21Room:4-ARack:CSentinel mouse program
2023/08/07Room:4-ARack:CAdditional tests
2023/05/22Room:4-ARack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)XEGFP human prepro-orexin promoter
F2A foot-and-mouth disease virus (FMD) 2A peptideXF2A
Prm protamine intron, polyA (mouse)XPrm
cre Phage P1 Cre recombinaseXcre

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman prepro-orexin promoter, jellyfish EGFP cDNA, Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Phage P1 Cre, mouse protamine intron and polyA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For profit use of the BIOLOGICAL RESSOURCE, the RECIPIENT must contact with the Intellectual Property Office, Nagoya University to obtain a prior written consent.RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAkihiro Yamanaka (Nagoya University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data