Strain Information | |
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Image | |
BRC No. | RBRC09240 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | STOCK Zeb2<tm2.1Yhi> |
Former Common name | SIP1-EGFP, Zfhx1b/GFP KI, STOCK Zfhx1b<tm2.1Yhi> |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Yujiro Higashi, Aichi Human Service Center in 2008. R1 ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with CAG-Cre mice (B6). ICR mixed background (RBRC09239), ICR and C57BL/6 mixed background (RBRC09240). |
Strain description | SIP1 (Zeb2, Zfhx1b) gene GFP knock-in mice. A EGFP was inserted in-frame with the coding sequence in exon 9 of the Zeb2 gene. |
Colony maintenance | Heterozygote x Wild-type |
References | SIP1 expression patterns in brain investigated by generating a SIP1-EGFP reporter knock-in mouse. Nishizaki Y, Takagi T, Matsui F, Higashi Y Genesis, 52, 56-67 (2014). 24243579 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | 2 | EGFP | ||||
Zeb2 MGI:1344407 | zinc finger E-box binding homeobox 2 | 2 | Zeb2<tm2.1Yhi> MGI:5574184 | targeted mutation 2.1, Yujiro Higashi | |||
loxP | phage P1 loxP | 2 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP site, Jellyfish EGFP cDNA, mouse Zeb2 genomic DNA |
Research application | Cre/loxP system Developmental Biology Research Fluorescent Proteins/lacZ System Mouse Models for Human Disease |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 52, 56-67 (2014). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Yujiro Higashi (Aichi Prefectural Colony) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Kim S, Chen J, Jo S, Ou F, Ferris ST, Liu TT, Ohara RA, Anderson DA, Wu R, Chen MY, Gillanders WE, Gillanders WE, Murphy TL, Murphy KM. IL-6 selectively suppresses cDC1 specification via C/EBPβ. J Exp Med 220(10) (2023) 37432392 |