Strain Data Sheet

RBRC09241

Strain Information

Image
BRC No.RBRC09241
TypeTransgene Congenic and Spontaneous MutationCartagena
SpeciesMus musculus
Strain nameHR1.Cg-Hr<hr> Tg(EF1A-PDHL/roGFP1)121Soht
Former Common nameMito-roGFP(MROH121), HR1-hr/Mito-roGFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Alexander M. Wolf, Nippon Medical School in 2012. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The mutant mice were backcrossed to Hos:HR-1 over 10 generations.
Strain descriptionTransgenic mice expressing ratiometric redox-sensitive GFP (roGFP) in the mitochondria of several tissues, including skin epidermal keratinocytes. Hairless mice background.
Colony maintenanceTg: Carrier x Noncarrier; hr: Homozygote x Homozygote [or Crossing to Hos:HR-1]
References
Real-time monitoring of oxidative stress in live mouse skin.
Wolf A M, Nishimaki K, Kamimura N, Ohta S
J. Invest. Dermatol., 134(6), 1701-1709 (2014). 24129062

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hr
MGI:96223		lysine demethylase and nuclear receptor corepressor14Hr<hr>
MGI:1856057		hairless
  • alopecia universalis congenita(DisGeNET, MedGEN)

  • atrichia with papular lesions(DisGeNET, MedGEN)
    • CSF3 human G-CSF cDNA PolyA signalUNCSF3
    PDHA1 mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence)UNPDHA1 human elongation factor 1 alpha (EF-1 alpha) promoter
    roGFP Redox-sensitive Green Fluorescent Protein (Aequorea victoria)UNroGFP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal dermis reticular layer morphology(MP:0005081)

  • abnormal mammary gland morphology(MP:0000627)

  • abnormal nursing(MP:0001382)

  • abnormal skin morphology(MP:0002060)

  • absent linear vestibular evoked potential(MP:0004813)
    • more 11 phenotypes
  • decreased T cell number(MP:0005018)

  • decreased T cell proliferation(MP:0005095)

  • deformed nails(MP:0000580)

  • dermal cyst(MP:0001247)

  • epidermal hyperplasia(MP:0001222)

  • hairless(MP:0003815)

  • hyperkeratosis(MP:0001242)

  • increased leukemia incidence(MP:0002026)

  • increased macrophage cell number(MP:0005425)

  • normal phenotype(MP:0002873)

  • sebaceous gland atrophy(MP:0000649)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman EF1A promoter, mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence, PDHA1), jellyfish GFP(EGFP) cDNA, human G-CSF cDNA PolyA signal
    Research applicationDermatology Research
    Fluorescent Proteins/lacZ System
    Metabolism Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol.,134(6), 1701-1709 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShigeo Ohta (Nippon Medical School)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data