Strain Data Sheet

RBRC09256

Strain Information

Image
BRC No.RBRC09256
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm1.1(CAG-kikGR)Kgwa>
Former Common nameB6-ROSA/kikGR KI, B6-ROSA/kikGR KI
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Michio Tomura, Kyoto University Graduate School of Medicine. M1 ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with CAG-Cre mice. C57BL/6 congenic.
Strain descriptiongreen-to-red photoconvertible fluorescence protein, KikGR ROSA26 knock-in mice.
Colony maintenanceHomozygote x Homozygote [or crossing to C57BL/6JJcl]
References
Tracking and quantification of dendritic cell migration and antigen trafficking between the skin and lymph nodes.
Tomura M, Hata A, Matsuoka S, Shand F H, Nakanishi Y, Ikebuchi R, Ueha S, Tsutsui H, Inaba K, Matsushima K, Miyawaki A, Kabashima K, Watanabe T, Kanagawa O
Sci. Rep., e6030 (2014). 25112380

Health Report

Examination Date / Room / Rack2025/01/07Room:3-BRack:CSentinel mouse program
2024/10/07Room:3-BRack:CSentinel mouse program
2024/07/08Room:3-BRack:CSentinel mouse program
2024/04/08Room:3-BRack:CSentinel mouse program
2024/01/09Room:3-BRack:CSentinel mouse program
2023/10/10Room:3-BRack:CSentinel mouse program
2023/07/10Room:3-BRack:CSentinel mouse program
2023/04/10Room:3-BRack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-kikGR)Kgwa> targeted mutation 1.1, Osami Kanagawa
kikGR Green-Red Fluorescent Protein (Favia favus)6kikGR CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxP phage P1 loxP6loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP site, Favia favus Kikume-Green Red cDNA, mouse Gt(ROSA)26Sor genomic region
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., e6030 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must send the DEPOSITOR a reprint of the RECIPIENT's publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing KikGR, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. PLoS One 3 (12): e3944 (2008).
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Zhang X, Huang N, Mu Y, Chen H, Zhu M, Zhang S, Liu P, Zhang H, Deng H, Feng K, Shang Q, Liu X, Zhang C, Shi M, Yang L, Sun J, Kong G, Geng J, Lu S, Li Z.
    Mechanical Force-Induced cGAS Activation in Carcinoma Cells Facilitates Splenocytes into Liver to Drive Metastasis.
    Adv Sci (Weinh) 12(8) e2401127(2025) 39737867