Strain Data Sheet

RBRC09257

Strain Information

Image
BRC No.RBRC09257
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC.Cg-Tg(CAG-tdKaede)15Utr
Former Common nameKaede (WK-m15), BALB-CAG-Kaede Tg#15
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Michio Tomura, Kyoto University Graduate School of Medicine and Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Atsushi Miyawaki, RIKEN Brain Science Institute. The Tg mice were backcrossed to BALB/c over 22 times.
Strain descriptionThese transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light.
Colony maintenanceCarrier x Noncarrier [BALB/cAJcl]
References
Monitoring cellular movement in vivo with photoconvertible fluorescence protein "Kaede" transgenic mice.
Tomura M, Yoshida N, Tanaka J, Karasawa S, Miwa Y, Miyawaki A, Kanagawa O
Proc. Natl. Acad. Sci. USA, 105(31):10871-10876 (2008). 18663225

Health Report

Examination Date / Room / Rack2024/11/25Room:4-BRack:ASentinel mouse program
2024/08/26Room:4-BRack:ASentinel mouse program
2024/05/27Room:4-BRack:ASentinel mouse program
2024/02/26Room:4-BRack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
tdKaede Trachyphyllia geoffroy Kaede cDNAUNtdKaede CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
SV40 polyA signalUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Trachyphyllia geoffroy Kaede cDNA, SV40 Poly(A) site
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 10871-10876 (2008). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Kaede, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Proc. Natl. Acad. Sci. USA, 99(20) 12651–12656 (2002).
    DepositorYoshihiro Miwa (University of Tsukuba)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Dec 2020

    BRC mice in Publications

    No Data