Strain Data Sheet

RBRC09303

Strain Information

Image
BRC No.RBRC09303
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.129(B6)-Chst15<tm1Oh>(BALB/cCrSlc)
Former Common nameGalNAc4S-6ST KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainBALB/cCrSlc
Appearance
Strain development
Strain descriptionExon 2 was replaced with a PGK-neo cassette.
Colony maintenance
References
Mice deficient in N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase are unable to synthesize chondroitin/dermatan sulfate containing N-acetylgalactosamine 4,6-bissulfate residues and exhibit decreased protease activity in bone marrow-derived mast cells.
Ohtake-Niimi S, Kondo S, Ito T, Kakehi S, Ohta T, Habuchi H, Kimata K, Habuchi O
J. Biol. Chem., 285, 20793-20805 (2010). 20439988

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Chst15
MGI:1924840		carbohydrate sulfotransferase 157Chst15<tm1Oh>
MGI:4840254		targeted mutation 1, Osami Habuchi
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal mast cell morphology(MP:0000359)

  • decreased litter size(MP:0001935)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, mouse GalNAc4S-6ST (CHST15) genomic DNA
    Research applicationMouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 285, 20793-20805 (2010). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorOsami Habuchi (Aichi Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data