Strain Data Sheet

RBRC09364

Strain Information

Image
BRC No.RBRC09364
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Igs7<tm92.1(tetO-YCX2.60)Hze>/J
Former Common nameAi92(TITL-YCX2.60)
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Madisen L, Garner A R, Shimaoka D, Chuong A S, Klapoetke N C, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen T N, Sunkin S M, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson R M, Knopfel T, Boyden E S, Reid R C, Carandini M, Zeng H
Neuron., 85(5): 942-958 (2015). 25741722

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
F3 yeast FRT (flippase recombination target) site (synthetic) variant9F3
F5 yeast FRT (flippase recombination target) site (synthetic) variant9F5
GH Growth hormone polyA (Bovine)9GH
GH Growth hormone polyA (Bovine)9GH
Hyg hygromycin phasphotransferase (E. coli)9Hyg mouse phosphoglycerate kinase promoter (PGK promoter)
Igs7
MGI:5490612
intergenic site 79Igs7<tm92.1(tetO-YCX2.60)Hze> targeted mutation 92.1, Hongkui Zeng
WPRE Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)9WPRE
YCX2.60 chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated ECFP (cpECFP) cDNA, rat calmodulin (CaM) cDNA, circularly permutated Venus enhanced yellow fluorescent protein cDNA9YCX2.60
loxP phage P1 loxP9loxP
loxP phage P1 loxP9loxP
SV40 polyA9

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
  • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) site FRT3/FRT5, chicken β-globin HS4 insulater, E. coli tetO promoter, P1 phage loxP sites, synthetic polyA, human hGH polyA, mouse PGK polyA, jellyfish CFP/YFP cDNA, Xenopus CaM, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine bGH polyA, E. coli AttB, mouse PGK promoter, E. coli hygromycin resistance gene, SV40 polyA, λphage AttP
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron., 85(5): 942-958 (2015). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAtsushi Miyawaki (RIKEN CBS)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Sequence of YCX2.60

    BRC mice in Publications

    No Data