Strain Data Sheet
RBRC09365
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC09365 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | STOCK Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>Igs7<tm92.1(tetO-YCX2.60)Hze>/J |
| Former Common name | ROSA26-ZtTA;Ai92(TITL-YCX2.60) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Visualizing the distribution of synapses from individual neurons in the mouse brain. Li L, Tasic B, Micheva K D, Ivanov V M, Spletter M L, Smith S J, Luo L PLoS One., 5(7):e11503 (2010). 20634890Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Madisen L, Garner A R, Shimaoka D, Chuong A S, Klapoetke N C, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen T N, Sunkin S M, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson R M, Knopfel T, Boyden E S, Reid R C, Carandini M, Zeng H Neuron., 85(5): 942-958 (2015). 25741722 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo> MGI:4461408 | targeted mutation 5, Liqun Luo | |||
| b-geo | lacZ reporter gene and neomycin resistance gene | 6 | b-geo | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| tTA | tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain) | 6 | tTA | ||||
| SV40 polyA | 6 | ||||||
| F3 | yeast FRT (flippase recombination target) site (synthetic) variant | 9 | F3 | ||||
| F5 | yeast FRT (flippase recombination target) site (synthetic) variant | 9 | F5 | ||||
| GH | Growth hormone polyA (Bovine) | 9 | GH | ||||
| GH | Growth hormone polyA (Bovine) | 9 | GH | ||||
| Hyg | hygromycin phasphotransferase (E. coli) | 9 | Hyg | mouse phosphoglycerate kinase promoter (PGK promoter) | |||
| Igs7 MGI:5490612 | intergenic site 7 | 9 | Igs7<tm92.1(tetO-YCX2.60)Hze> | targeted mutation 92.1, Hongkui Zeng | |||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | 9 | WPRE | ||||
| YCX2.60 | chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated ECFP (cpECFP) cDNA, rat calmodulin (CaM) cDNA, circularly permutated Venus enhanced yellow fluorescent protein cDNA | 9 | YCX2.60 | ||||
| loxP | phage P1 loxP | 9 | loxP | ||||
| loxP | phage P1 loxP | 9 | loxP | ||||
| SV40 polyA signal | 9 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Cytomegalovirus CMV enhancer, chicken β-actin promoter, P1 phage loxP sites, E. coli β-galactosidase, E. coli neomycin-resistance gene, Tet transactivator (E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain), SV40 polyA,/ yeast FRT (flipase recombination target) sites (FRT3 and FRT5), chicken β-globin HS4 insulater, E. coli tetO promoter, synthetic polyA, human hGH polyA, mouse PGK polyA, jellyfish CFP/YFP cDNA, Xenopus CaM, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine bGH polyA, E. coli AttB, λphage AttP, mouse PGK promoter, E. coli hygromycin resistance gene |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron., 85(5): 942-958 (2015). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Atsushi Miyawaki (RIKEN CBS) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Sequence of YCX2.60 |
BRC mice in Publications |
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| No Data |