Strain Data Sheet

RBRC09390

Strain Information

Image
BRC No.RBRC09390
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Nkapl<tm1(KOMP)Vlcg>/1Htan
Former Common nameNkapl KO
H-2 Haplotype
ES Cell lineVGB6 [C57BL/6NTac]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
A novel transcriptional factor Nkapl is a germ cell-specific suppressor of Notch signaling and is indispensable for spermatogenesis.
Okuda H, Kiuchi H, Takao T, Miyagawa Y, Tsujimura A, Nonomura N, Miyata H, Okabe M, Ikawa M, Kawakami Y, Goshima N, Wada M, Tanaka H
PLoS One 10, e0124293 (2015). 25875095

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nkapl
MGI:1913957		NFKB activating protein-like13Nkapl<tm1(KOMP)Vlcg>
MGI:4415546		targeted mutation 1, Velocigene
lacZ beta-galactosidase (E. coli)13
loxP phage P1 loxP13loxP
loxP phage P1 loxP13loxP
neo neomycin resistance gene (E. coli)13 human Ubiquitin C promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • arrest of male meiosis(MP:0008261)

  • azoospermia(MP:0005159)

  • increased male germ cell apoptosis(MP:0014052)

  • male infertility(MP:0001925)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAVelocigene cassette ZEN-Ub1(E. coli LacZ-p(A), Phage P1 loxP sites, human Ubiquitin C promoter, E. coli Neomycin resistance gene, polyA), mouse Nkapl genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The mice were originally created by the National Institutes of Health (NIH) Knock-Out Mouse Program (KOMP). The RECIPIENT is free to publish, present, display, disclose or seek patent or other intellectual property protection on any inventions arising from the use of the KOMP Materials, with no restrictions or obligations of any kind, so long as the RECIPIENT uses reasonable efforts to credit or otherwise acknowledge the KOMP as the creator of the KOMP Materials.
    DepositorHiromitsu Tanaka (Nagasaki International University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data