Strain Data Sheet

RBRC09393

Strain Information

Image
BRC No.RBRC09393
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Kcnj11<tm2Sse>
Former Common nameKir6.2-flox
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Susumu Seino, Kobe University Graduate School of Medicine. ES cells derived from C57BL/6 were used. C57BL/6 genetic background.
Strain description
Colony maintenance
References
Gs/Gq signaling switch in β cells defines incretin effectiveness in diabetes.
Okechi S Oduori, Naoya Murao, Kenju Shimomura, Harumi Takahashi, Quan Zhang, Haiqiang Dou, Shihomi Sakai, Kohtaro Minami, Belen Chanclon, Claudia Guida, Lakshmi Kothegala, Johan Tolö, Yuko Maejima, Norihide Yokoi, Yasuhiro Minami, Takashi Miki, Patrik Rorsman, Susumu Seino
J. Clin. Invest., 130(12):6639-6655 (2020). 33196462

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site7Frt
Frt yeast FRT (flippase recombination target) site7Frt
GH Growth hormone polyA (Bovine)7GH
Kcnj11
MGI:107501		potassium inwardly rectifying channel, subfamily J, member 117Kcnj11<tm2Sse> targeted mutation 2, Susumu Seino
  • diabetes mellitus, permanent neonatal 2(MedGEN)

  • diabetes mellitus, transient neonatal, 3(MedGEN)

  • hyperinsulinemic hypoglycemia, familial, 2(MedGEN)
    • more 3 Diseases
  • maturity-onset diabetes of the young type 13(MedGEN)

  • permanent neonatal diabetes mellitus(DisGeNET)

  • type 2 diabetes mellitus(DisGeNET, MedGEN)
    • loxP phage P1 loxP7loxP
    loxP phage P1 loxP7loxP
    neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, Yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Bovine Growth hormone poly A signal, mouse Kcnj11 genomic DNA
    Research applicationCre/loxP system
    Endocrine Deficiency Research
    FLP/frt system
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorSusumu Seino (Kobe University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data