Strain Data Sheet

RBRC09404

Strain Information

Image
BRC No.RBRC09404
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.CBA-Park2<tm1Tchi>
Former Common nameParkin KO, B6.CBA-Park2<tm1Tchi>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Decline of striatal dopamine release in parkin-deficient mice shown by ex vivo autoradiography.
Sato S, Chiba T, Nishiyama S, Kakiuchi T, Tsukada H, Hatano T, Fukuda T, Yasoshima Y, Kai N, Kobayashi K, Mizuno Y, Tanaka K, Hattori N
J. Neurosci. Res., 84:1350-1357 (2006). 16941649

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)17
MAPT microtubule-associated protein tau (human)17MAPT
Prkn
MGI:1355296		parkin RBR E3 ubiquitin protein ligase17Prkn<tm1Tchi>
MGI:6758222		targeted mutation 1, Tomoki Chiba
  • autosomal recessive juvenile Parkinson disease 2(DisGeNET, MedGEN)

  • lung cancer(MedGEN)

  • ovarian neoplasm(MedGEN)
    • loxP phage P1 loxP17loxP
    neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • increased susceptibility to dopaminergic neuron neurotoxicity(MP:0011451)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman MAPT cDNA, Aequorea victoria GFP cDNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse PGK pA, Phage P1 loxP site, mouse Prkn genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Fluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci. Res., 84:1350-1357 (2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact with the DEPOSITOR and discuss about collaboration prior to use the BIOLOGICAL RESOURCE.
    DepositorTomoki Chiba (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data