Strain Data Sheet

RBRC09422

Strain Information

Image
BRC No.RBRC09422
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameFVB.129S6-Gt(ROSA)26Sor<tm1(Pik3ca*E545K)Egan>
Former Common nameR26-Pik3ca(E545K)
H-2 Haplotype
ES Cell lineW4 [129S6/SvEvTac]
Background strainFVB/NJcl
Appearance
Strain developmentW4 mESC were transformed with linearized pROSA-Pik3ca-H1047R or pROSA-Pik3ca-E545K plasmid; this plasmid was built from pBIGT and pROSA gene targeting vectors and contained the mouse Pik3ca cDNA with H1047R or E545K point mutation.
Strain descriptionSome mice develop blood vessel lesions.
Colony maintenance
References
Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation.
Adams J R, Xu K, Liu J C, Agamez N M, Loch A J, Wong R G, Wang W, Wright K L, Lane T F, Zacksenhaus E, Egan S E
Cancer Res., 1, 2706-2717 (2011). 21324922

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (Bovine)6GH
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Pik3ca*E545K)Egan> targeted mutation 1, Sean E Egan
Pik3ca
MGI:1206581		phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha6Pik3ca
  • CLAPO syndrome(MedGEN)

  • CLOVES syndrome(DisGeNET, MedGEN)

  • colorectal cancer(MedGEN)
    • more 10 Diseases
  • Cowden syndrome 5(MedGEN)

  • gastric cancer(MedGEN)

  • hepatocellular carcinoma(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • lung cancer(MedGEN)

  • megalencephaly-capillary malformation-polymicrogyria syndrome(MedGEN)

  • megalodactyly(MedGEN)

  • nevus, epidermal(MedGEN)

  • ovarian neoplasm(MedGEN)

  • seborrheic keratosis(MedGEN)
    • SA Splice acceptor (adenovirus)6SA
    loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)
    tpA trimer of the SV40 polyadenylation sequence (tpA)6tpA

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • increased mammary gland tumor incidence(MP:0010299)

  • increased tumor latency(MP:0009828)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus splice acceptor (SA), Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, trimer of the SV40 polyadenylation sequence (tpA), mouse Pik3ca E545K cDNA, bovine growth hormone polyadenylation sequence (bpA), mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCancer Research
    Cre/loxP system
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must prior contact with the developer scientist(Dr. Sean E. Egan, segan@sickkids.ca, Hospital for Sick Children) and must obtain a use license.
    DepositorYasushi Okazaki (Saitama Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data