Strain Data Sheet

RBRC09426

Strain Information

Image
BRC No.RBRC09426
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Nepro<tm1(Venus)Tets> flox
Former Common nameB6.129-Nepro<Venus-neo>1
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Identification of Nepro, a gene required for the maintenance of neocortex neural progenitor cells downstream of Notch.
Muroyama Y, Saito T
Development, 136, 3889-3893 (2009). 19906856

Nepro is localized in the nucleolus and essential for preimplantation development in mice.
Hashimoto M, Sato T, Muroyama Y, Fujimura L, Hatano M, Saito T
Dev Growth Differ., 57(7):529-538 (2015). 26178919

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nepro
MGI:2384836		nucleolus and neural progenitor protein16Nepro<tm1(Venus)Tets> targeted mutation 1, Tetsuichiro Saito
  • anauxetic dysplasia 3(MedGEN)
    • Venus Yellow Fluorescent Protein (Aequorea victoria)16Venus
    loxP phage P1 loxP16loxP
    loxP phage P1 loxP16loxP
    neo neomycin resistance gene (E. coli)16 mouse phosphoglycerate kinase promoter (PGK promoter)
    SV40 polyA signal16

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cell nucleus morphology(MP:0003111)

  • abnormal nucleolus morphology(MP:0008830)

  • embryonic lethality before implantation, complete penetrance(MP:0011094)

  • failure of blastocyst formation(MP:0012129)
    • Detailed phenotype data

    Ordering Information

    Donor DNAjellyfish Venus cDNA, Phage P1 loxP sites, mouse Phosphoglycerate kinase (PGK) promoter, E. coli Neomycin resistance gene, SV40 polyA signal, mouse Nepro genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 136, 3889-3893 (2009). Development Growth and Differentiation, 57(7):529-538 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorTetsuichiro Saito (Chiba University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data