Strain Data Sheet

RBRC09428

Strain Information

Image
BRC No.RBRC09428
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Adarb1<tm1.1Skwa>Tg(SLC18A3-cre)KMisa
Former Common nameAR2; C57BL/6-ADAR2<flox/flox>/VAChT-Cre. Fast mouse
H-2 Haplotype
ES Cell lineLex-2 [129SvEvBrd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
VAChT-Cre. Fast and VAChT-Cre.Slow: postnatal expression of Cre recombinase in somatomotor neurons with different onset.
Misawa H, Nakata K, Toda K, Matsuura J, Oda Y, Inoue H, Tateno M, Takahashi R
Genesis, 37, 44-50 (2003). 14502577

Induced loss of ADAR2 engenders slow death of motor neurons from Q/R site-unedited GluR2.
Hideyama T, Yamashita T, Suzuki T, Tsuji S, Higuchi M, Seeburg P H, Takahashi R, Misawa H, Kwak S
J. Neurosci., 30, 11917-11925 (2010). 20826656

Health Report

Examination Date / Room / Rack2024/11/25Room:4-CRack:GSentinel mouse program
2024/08/26Room:4-CRack:GSentinel mouse program
2024/05/27Room:4-CRack:GSentinel mouse program
2024/02/26Room:4-CRack:GSentinel mouse program
2023/11/27Room:4-CRack:GSentinel mouse program
2023/08/28Room:4-CRack:GSentinel mouse program
2023/05/29Room:4-CRack:GSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Adarb1
MGI:891999		adenosine deaminase, RNA-specific, B110Adarb1
  • neurodevelopmental disorder with hypotonia, microcephaly, and seizures(MedGEN)
    • Frt yeast FRT (flippase recombination target) site10Frt
    Frt yeast FRT (flippase recombination target) site10Frt
    loxP phage P1 loxP10loxP
    loxP phage P1 loxP10loxP
    neo neomycin resistance gene (E. coli)10 mouse phosphoglycerate kinase promoter (PGK promoter)
    GFP Green Fluorescent Protein (Aequorea victoria)UN
    IRES internal ribosomal entry site (EMCV)UN
    cre Phage P1 Cre recombinaseUNcre human vesicular acetylcholine transporter

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cranial nerve morphology(MP:0001056)

  • abnormal facial nerve morphology(MP:0001071)

  • abnormal hypoglossal nerve morphology(MP:0001076)

  • abnormal innervation(MP:0002184)

  • abnormal limb posture(MP:0004263)
    • more 14 phenotypes
  • abnormal neuromuscular synapse morphology(MP:0001053)

  • abnormal posture(MP:0001504)

  • abnormal skeletal muscle fiber morphology(MP:0003084)

  • abnormal ventral spinal root morphology(MP:0003993)

  • behavior/neurological phenotype(MP:0005386)

  • centrally nucleated skeletal muscle fibers(MP:0009404)

  • decreased body weight(MP:0001262)

  • decreased grip strength(MP:0010053)

  • decreased locomotor activity(MP:0001402)

  • decreased spinal cord ventral horn cell number(MP:0005113)

  • impaired coordination(MP:0001405)

  • motor neuron degeneration(MP:0000938)

  • premature death(MP:0002083)

  • skeletal muscle fiber atrophy(MP:0009413)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Adarb1 genomic DNA, Human vesicular acetylcholine transporter (SLC18A) genomic DNA, Phage P1 Cre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Jellyfish GFP cDNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci, 30, 11917-11925 (2010).
    DepositorKeiji Itaka (The University of Tokyo)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Nov 2015

    BRC mice in Publications

    No Data