Strain Data Sheet

RBRC09435

Strain Information

Image
BRC No.RBRC09435
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Gt(ROSA)26Sor<tm1(Stop-3xFlag ESET,EGFP)>
Former Common nameRosa26-3xFlag-ESET-IRES-GFP- KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)6EGFP
ESET 6ESET
FLAG FLAG tag (synthetic)6FLAG
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Stop-3xFlag ESET,EGFP)> targeted mutation 1, Atsushi Iwama
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
neo neomycin resistance gene (E. coli)6 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Stop (synthetic), FLAG tag (synthetic), mouse ESET cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Aequorea victoria EGFP cDNA, mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorAtsushi Iwama (Chiba University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data