Strain Data Sheet

RBRC09446

Strain Information

Image
BRC No.RBRC09446
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Islr<tm1a(EUCOMM)Wtsi>/Ea
Former Common nameIslr KO
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Cloning of the cDNA for a new member of the immunoglobulin superfamily (ISLR) containing leucine-rich repeat (LRR).
Nagasawa A, Kubota R, Imamura Y, Nagamine K, Wang Y, Asakawa S, Kudoh J, Minoshima S, Mashima Y, Oguchi Y, Shimizu N
Genomics, 44, 273-279 (1997). 9325048

Identification of Meflin as a Potential Marker for Mesenchymal Stromal Cells.
Maeda K, Enomoto A, Hara A, Asai N, Kobayashi T, Horinouchi A, Maruyama S, Ishikawa Y, Nishiyama T, Kiyoi H, Kato T, Ando K, Weng L, Mii S, Asai M, Mizutani Y, Watanabe O, Hirooka Y, Goto H, Takahashi M
Sci. Rep., 6:22288 (2016). 26924503

Roles of the Mesenchymal Stromal/Stem Cell Marker Meflin in Cardiac Tissue Repair and the Development of Diastolic Dysfunction.
Hara A, Kobayashi H, Asai N, Saito S, Higuchi T, Kato K, Okumura T, Bando Y K, Takefuji M, Mizutani Y, Miyai Y, Saito S, Maruyama S, Maeda K, Ouchi N, Nagasaka A, Miyata T, Mii S, Kioka N, Worthley D L, Murohara T, Takahashi M, Enomoto A
Circ. Res.,125(4):414-430 (2019). 31221024

Meflin-Positive Cancer-Associated Fibroblasts Inhibit Pancreatic Carcinogenesis.
Mizutani Y, Kobayashi H, Iida T, Asai N, Masamune A, Hara A, Esaki N, Ushida K, Mii S, Shiraki Y, Ando K, Weng L, Ishihara S, Ponik S M, Conklin M W, Haga H, Nagasaka A, Miyata T, Matsuyama M, Kobayashi T, Fujii T, Yamada S, Yamaguchi J, Wang T, Woods S L, Worthley D, Shimamura T, Fujishiro M, Hirooka Y, Enomoto A, Takahashi M
Cancer Res., 79(20):5367-5381 (2019). 31439548

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)9En2
Frt yeast FRT (flippase recombination target) site9Frt
Frt yeast FRT (flippase recombination target) site9Frt
Ires internal ribosomal entry site (EMCV)9
Islr
MGI:1349645
immunoglobulin superfamily containing leucine-rich repeat9Islr<tm1a(EUCOMM)Wtsi>
MGI:5006757
targeted mutation 1a, Wellcome Trust Sanger Institute
SA Splice acceptor9SA
lacZ beta-galactosidase (E. coli)9
loxP phage P1 loxP9loxP
loxP phage P1 loxP9loxP
loxP phage P1 loxP9loxP
neo neomycin resistance gene (E. coli)9 human beta-actin promoter
SV40 polyA signal9

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal bone marrow cell morphology/development(MP:0002398)

  • abnormal osteoblast morphology(MP:0004986)

  • abnormal osteoid morphology(MP:0010924)

  • decreased testis weight(MP:0004852)

  • hematopoietic system phenotype(MP:0005397)
  • more 6 phenotypes
  • increased osteoblast cell number(MP:0004988)

  • increased osteoid volume(MP:0010926)

  • long radius(MP:0008951)

  • long tibia(MP:0004357)

  • skeleton phenotype(MP:0005390)

  • slow postnatal weight gain(MP:0008489)
  • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Islr genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 6:22288 (2016). Circ. Res.,125(4):414-430 (2019). Cancer Res., 79(20):5367-5381 (2019).This strain was established by the DEPOSITOR by using knockout ES cells of EUCOMM Consortium. The RECIPIENT shall acknowledge the EUCOMM Consortium as the source of the ES cells and the DEPOSITOR for providing the strain. The strain shall only be used for non-commercial purposes.
    DepositorAtsushi Enomoto (Nagoya University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jun 2022

    BRC mice in Publications

    No Data