Strain Information | |
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Image | |
BRC No. | RBRC09446 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | C57BL/6-Islr<tm1a(EUCOMM)Wtsi>/Ea |
Former Common name | Islr KO |
H-2 Haplotype | |
ES Cell line | JM8A3.N1 [C57BL/6N-A<tm1Brd>] |
Background strain | |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References | Cloning of the cDNA for a new member of the immunoglobulin superfamily (ISLR) containing leucine-rich repeat (LRR). Nagasawa A, Kubota R, Imamura Y, Nagamine K, Wang Y, Asakawa S, Kudoh J, Minoshima S, Mashima Y, Oguchi Y, Shimizu N Genomics, 44, 273-279 (1997). 9325048Identification of Meflin as a Potential Marker for Mesenchymal Stromal Cells. Maeda K, Enomoto A, Hara A, Asai N, Kobayashi T, Horinouchi A, Maruyama S, Ishikawa Y, Nishiyama T, Kiyoi H, Kato T, Ando K, Weng L, Mii S, Asai M, Mizutani Y, Watanabe O, Hirooka Y, Goto H, Takahashi M Sci. Rep., 6:22288 (2016). 26924503Roles of the Mesenchymal Stromal/Stem Cell Marker Meflin in Cardiac Tissue Repair and the Development of Diastolic Dysfunction. Hara A, Kobayashi H, Asai N, Saito S, Higuchi T, Kato K, Okumura T, Bando Y K, Takefuji M, Mizutani Y, Miyai Y, Saito S, Maruyama S, Maeda K, Ouchi N, Nagasaka A, Miyata T, Mii S, Kioka N, Worthley D L, Murohara T, Takahashi M, Enomoto A Circ. Res.,125(4):414-430 (2019). 31221024Meflin-Positive Cancer-Associated Fibroblasts Inhibit Pancreatic Carcinogenesis. Mizutani Y, Kobayashi H, Iida T, Asai N, Masamune A, Hara A, Esaki N, Ushida K, Mii S, Shiraki Y, Ando K, Weng L, Ishihara S, Ponik S M, Conklin M W, Haga H, Nagasaka A, Miyata T, Matsuyama M, Kobayashi T, Fujii T, Yamada S, Yamaguchi J, Wang T, Woods S L, Worthley D, Shimamura T, Fujishiro M, Hirooka Y, Enomoto A, Takahashi M Cancer Res., 79(20):5367-5381 (2019). 31439548 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
En2 | En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence) | 9 | En2 | ||||
Frt | yeast FRT (flippase recombination target) site | 9 | Frt | ||||
Frt | yeast FRT (flippase recombination target) site | 9 | Frt | ||||
Ires | internal ribosomal entry site (EMCV) | 9 | |||||
Islr MGI:1349645 | immunoglobulin superfamily containing leucine-rich repeat | 9 | Islr<tm1a(EUCOMM)Wtsi> MGI:5006757 | targeted mutation 1a, Wellcome Trust Sanger Institute | |||
SA | Splice acceptor | 9 | SA | ||||
lacZ | beta-galactosidase (E. coli) | 9 | |||||
loxP | phage P1 loxP | 9 | loxP | ||||
loxP | phage P1 loxP | 9 | loxP | ||||
loxP | phage P1 loxP | 9 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 9 | human beta-actin promoter | ||||
SV40 polyA signal | 9 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 6 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Islr genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 6:22288 (2016). Circ. Res.,125(4):414-430 (2019). Cancer Res., 79(20):5367-5381 (2019).This strain was established by the DEPOSITOR by using knockout ES cells of EUCOMM Consortium. The RECIPIENT shall acknowledge the EUCOMM Consortium as the source of the ES cells and the DEPOSITOR for providing the strain. The strain shall only be used for non-commercial purposes. |
Depositor | Atsushi Enomoto (Nagoya University) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jun 2022 |
BRC mice in Publications |
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