Strain Data Sheet
RBRC09446
Strain Information | |
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| Image | |
| BRC No. | RBRC09446 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Islr<tm1a(EUCOMM)Wtsi>/Ea |
| Former Common name | Islr KO |
| H-2 Haplotype | |
| ES Cell line | JM8A3.N1 [C57BL/6N-A<tm1Brd>] |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Genomics, 44, 273-279 (1997). 9325048 Circ. Res.,125(4):414-430 (2019). 31221024 Cancer Res., 79(20):5367-5381 (2019). 31439548 Sci. Rep., 6:22288 (2016). 26924503 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Islrimmunoglobulin superfamily containing leucine-rich repeat9Islr<tm1a(EUCOMM)Wtsi>targeted mutation 1a, Wellcome Trust Sanger InstituteMeflin Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)9En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site9Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site9Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)9 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor9SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)9 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9human beta-actin promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal9 |
Ordering Information | |
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| Donor DNA | yeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Islr genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 6:22288 (2016). Circ. Res.,125(4):414-430 (2019). Cancer Res., 79(20):5367-5381 (2019).This strain was established by the DEPOSITOR by using knockout ES cells of EUCOMM Consortium. The RECIPIENT shall acknowledge the EUCOMM Consortium as the source of the ES cells and the DEPOSITOR for providing the strain. The strain shall only be used for non-commercial purposes. |
| Depositor | Atsushi Enomoto (Nagoya University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |