Strain Data Sheet
RBRC09450
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC09450 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6-Gt(Rosa)26Sor<tm1(CAG-GCaMP6,-mCherry)Shi> |
| Former Common name | RCL-G6-2A-mCh |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J PLoS One, 7, e51286, (2012). 23240011 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/12/09Room:3-2Rack:ASentinel mouse program 2024/09/09Room:3-2Rack:ASentinel mouse program 2024/06/10Room:3-2Rack:ASentinel mouse program 2024/03/11Room:3-2Rack:ASentinel mouse program 2023/12/11Room:3-2Rack:ASentinel mouse program 2023/09/11Room:3-2Rack:ASentinel mouse program 2023/06/26Room:3-2Rack:AAdditional tests 2023/06/13Room:3-2Rack:ASentinel mouse program 2023/03/13Room:3-2Rack:ASentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| F2A | foot-and-mouth disease virus (FMD) 2A peptide | 6 | F2A | ||||
| GCaMP6 | RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA | 6 | GCaMP6 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1(CAG-GCaMP6,-mCherry)Shi> | targeted mutation 1, Shionogi | |||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | 6 | WPRE | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| mCherry | Red Fluorescent Protein (Discosoma sp.) | 6 | mCherry | ||||
| neo | neomycin resistance gene (E. coli) | 6 | |||||
| poly A | polyadenylic acid additional signal | 6 | poly A | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage P1 LoxP sites, E. coli Neomycin resistance gene, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Discosoma sp. mCherry cDNA, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element(WPRE), polyA, mouse Gt(Rosa)26Sor genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | 1) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. 2) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. 3) The BIOLOGICAL RESOURCE must not be provided to any third party without DEPOSITOR’s permission. 4) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2012 Dec 11; 7(12), e51286, 2012. |
| Depositor | Shionogi & Co., Ltd. (Shionogi & Co., Ltd) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |