Strain Data Sheet

RBRC09451

Strain Information

Image
BRC No.RBRC09451
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB-Tg(Thy1-GCaMP6-actin/mCherry)1Shi
Former Common nameFVB-Thy1-G6-actin-mCherry-Tg #6
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals.
Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J
PLoS One, 7, e51286, (2012). 23240011

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
F2A foot-and-mouth disease virus (FMD) 2A peptideUNF2A
GCaMP6-actin RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA, linker, human beta actin cDNAUNGCaMP6-actin Thy1 promoter
WPRE Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE
mCherry Red Fluorescent Protein (Discosoma sp.)UNmCherry
poly A polyadenylic acid additional signalUNpoly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Thy1,2 Expression cassette, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), Human β-actin, Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Discosoma sp. mCherry cDNA, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element(WPRE)
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and Conditions1) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    2) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    3) The BIOLOGICAL RESOURCE must not be provided to any third party without DEPOSITOR’s permission.
    4) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 7, e51286, (2012).
    DepositorShionogi & Co., Ltd. (Shionogi & Co., Ltd)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: FP license of TBUSA
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data