Strain Data Sheet

RBRC09452

Strain Information

Image
BRC No.RBRC09452
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB-Tg(Thy1-GCaMP6)5Shi
Former Common nameFVB-Thy1-G6-Tg #5
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals.
Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J
PLoS One, 7, e51286, (2012). 23240011

Confocal and multiphoton calcium imaging of the enteric nervous system in anesthetized mice.
Motegi Y, Sato M, Horiguchi K, Ohkura M, Gengyo-Ando K, Ikegaya Y, Fusamae Y, Hongo Y, Suzuki M, Ogawa K, Takaki M, Nakai J
Neurosci. Res., 151:53-60 (2019). 30790590

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GCaMP6 RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP6 Thy1 promoter
WPRE Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE
poly A polyadenylic acid additional signalUNpoly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Thy1,2 Expression cassette, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element(WPRE)
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and Conditions1) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    2) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    3) The BIOLOGICAL RESOURCE must not be provided to any third party without DEPOSITOR’s permission.
    4) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 7, e51286, (2012).
    DepositorShionogi & Co., Ltd. (Shionogi & Co., Ltd)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Castillo VCG, Akbar L, Siwadamrongpong R, Ohta Y, Kawahara M, Sunaga Y, Takehara H, Tashiro H, Sasagawa K, Ohta J.
    Region of interest determination algorithm of lensless calcium imaging datasets.
    PLoS One 19(9) e0308573(2024) 39288120
    Donaldson PD, Navabi ZS, Carter RE, Fausner SML, Ghanbari L, Ebner TJ, Swisher SL, Kodandaramaiah SB.
    Polymer Skulls With Integrated Transparent Electrode Arrays for Cortex-Wide Opto-Electrophysiological Recordings.
    Adv Healthc Mater 11(18) e2200626(2022) 35869830