Strain Data Sheet

RBRC09454

Strain Information

Image
BRC No.RBRC09454
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129S.Cg-Unc5d<tm1.1>
Former Common name129-Unc5D KO
H-2 Haplotype
ES Cell lineF1(C57BL/6J x 129/SvJ)
Background strain
Appearance
Strain developmentDeveloped by Dr. Seiki Haraguchi, National Agriculture and Food Research Organization.
Strain descriptionExon 1 was replaced with a loxP-neo-loxP cassette. The floxed neo cassette was removed by cre excision.
Colony maintenance
References
Dependence receptor UNC5D mediates nerve growth factor depletion-induced neuroblastoma regression.
Zhu Y, Li Y, Haraguchi S, Yu M, Ohira M, Ozaki T, Nakagawa A, Ushijima T, Isogai E, Koseki H, Nakamura Y, Kong C, Mehlen P, Arakawa H, Nakagawara A
Journal of Clinical Investigation., 123, 2935-2947 (2013). 23778138

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Unc5d
MGI:2389364		unc-5 netrin receptor D8Unc5d<tm1.1Shar> targeted mutation 1.1,Seiki Haraguchi
loxP phage P1 loxP8loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased neuron apoptosis(MP:0003204)

  • increased neuronal precursor cell number(MP:0004980)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP site, mouse Unc5d genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorYuichi Wakabayashi (Chiba Cancer Center)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data