Strain Data Sheet

RBRC09462

Strain Information

Image
BRC No.RBRC09462
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tc (CYP3A-MAC)-Cyp3a
Former Common nameC3M/BW, STOCK Tc (CYP3A-MAC)-Cyp3a
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Trans-chromosomic mice containing a human CYP3A cluster for prediction of xenobiotic metabolism in humans.
Kazuki Y, Kobayashi K, Aueviriyavit S, Oshima T, Kuroiwa Y, Tsukazaki Y, Senda N, Kawakami H, Ohtsuki S, Abe S, Takiguchi M, Hoshiya H, Kajitani N, Takehara S, Kubo K, Terasaki T, Chiba K, Tomizuka K, Oshimura M
Hum Mol Genet., 22(3), 578-592 (2013). 23125282

Highly stable maintenance of a mouse artificial chromosome in human cells and mice.
Kazuki K, Takehara S, Uno N, Imaoka N, Abe S, Takiguchi M, Hiramatsu K, Oshimura M, Kazuki Y
Biochem Biophys Res Commun., 442(1-2), 44-50 (2013). 24216103

A novel and stable mouse artificial chromosome vector.
Takiguchi M, Kazuki Y, Hiramatsu K, Abe S, Iida Y, Takehara S, Nishida T, Ohbayashi T, Wakayama T, Oshimura M
ACS Synth Biol., 3(12):903-914(2014). 23654256

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cyp3a13
MGI:88610		cytochrome P450, family 3, subfamily a, polypeptide 135Cyp3a13<tm1Mtos>
MGI:5469196		targeted mutation 1, Mitsuo Oshimura
CYP3A cytochrome P450, family 3, subfamily a cluster (human)UNCYP3A
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)EGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
GFP Green Fluorescent Protein (Aequorea victoria) mouse phosphoglycerate kinase promoter (PGK promoter)
hyg hygromycin phasphotransferase (E. coli)hyg mouse phosphoglycerate kinase promoter (PGK promoter)
loxP phage P1 loxPloxP
loxP phage P1 loxPloxP
loxP phage P1 loxPloxP
neo neomycin resistance gene (E. coli) mouse phosphoglycerate kinase promoter (PGK promoter)
neo neomycin resistance gene (E. coli)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal enzyme/coenzyme activity(MP:0005584)

  • infertility(MP:0001924)

  • male infertility(MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman CYP3A cluster genomic DNA, Bovine growth hormone (BGH) polyA signal, chicken beta-globin insulator, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 large antigen，SV40 early promoter, SV40 Origin (Ori), polyA signal, P1 Phage loxP site, E. coli ampicilin resistance gene, E. coli Kan resistance gene, E. coli hyg resistance gene, jellyfish GFP cDNA, mouse PGK promoter, mouse chromosome 11 centromere region
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT must inform the DEPOSITOR whether its research plan includes breeding, crossing with other strains and/or modifications of an inserted DNA. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for an academic research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorHiroyuki Kugoh (Tottori University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data