Strain Data Sheet

RBRC09475

Strain Information

Image
BRC No.RBRC09475
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Piwil2<tm1Nkn>
Former Common nameMili-KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Mili, a mammalian member of piwi family gene, is essential for spermatogenesis.
Kuramochi-Miyagawa S, Kimura T, Ijiri T W, Isobe T, Asada N, Fujita Y, Ikawa M, Iwai N, Okabe M, Deng W, Lin H, Matsuda Y, Nakano T
Development., 131, 839-849 (2004). 14736746

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Piwil2
MGI:1930036		piwi-like RNA-mediated gene silencing 214Piwil2<tm1Nkn>
MGI:3029596		targeted mutation 1, Toru Nakano
neo neomycin resistance gene (E. coli)14 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal male germ cell apoptosis(MP:0008280)

  • arrest of male meiosis(MP:0008261)

  • azoospermia(MP:0005159)

  • decreased testis weight(MP:0004852)

  • male infertility(MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, mouse Phosphoglycerate kinase (Pgk1) promoter and polyA, mouse Mili (mouse PiwiL2)genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development., 131, 839-849 (2004). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (by e-mail).
    DepositorToru Nakano (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data