Strain Data Sheet

RBRC09476

Strain Information

Image
BRC No.RBRC09476
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Piwil4<tm1Nkn>
Former Common nameMiwi2-KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes.
Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Totoki Y, Toyoda A, Ikawa M, Asada N, Kojima K, Yamaguchi Y, Ijiri T W, Hata K, Li E, Matsuda Y, Kimura T, Okabe M, Sakaki Y, Sasaki H, Nakano T
Genes Dev., 1, 908-917 (2008). 18381894

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Piwil4
MGI:3041167		piwi-like RNA-mediated gene silencing 49Piwil4<tm1Nkn> targeted mutation 1, Toru Nakano
neo neomycin resistance gene (E. coli)9 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • reduced male fertility(MP:0001922)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, mouse Phosphoglycerate kinase(Pgk1) promoter and polyA, mouse Miwi2 (mouse PiwiL4) genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 1, 908-917 (2008). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (by e-mail).
    DepositorToru Nakano (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data