Strain Data Sheet

RBRC09477

Strain Information

Image
BRC No.RBRC09477
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameBALB/c-Ryr2<tm1Nobs>
Former Common nameBALB/c-Ryr2<tm1Nobs>
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strainBALB/cCrSlc
Appearance
Strain development
Strain description
Colony maintenance
References
Cooperation of oncogenic K-ras and p53 deficiency in pleomorphic rhabdomyosarcoma development in adult mice.
Tsumura H, Yoshida T, Saito H, Imanaka-Yoshida K, Suzuki N
Oncogene., 25, 7673-7679 (2006). 16785989

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (Bovine)13GH
KRAS Kirsten rat sarcoma viral oncogene homolog (KRAS)13KRAS
Ryr2
MGI:99685		ryanodine receptor 2, cardiac13Ryr2<tm1(CAG-KRAS*G12V,-lacZ)Nobs>
MGI:3698416		targeted mutation 1, Noboru Suzuki
  • catecholaminergic polymorphic ventricular tachycardia 1(DisGeNET, MedGEN)

  • obsolete arrhythmogenic right ventricular dysplasia 2(MedGEN)

  • ventricular arrhythmias due to cardiac ryanodine receptor calcium release deficiency syndrome(MedGEN)
    • lacZ beta-galactosidase (E. coli)13
    loxP phage P1 loxP13loxP
    loxP phage P1 loxP13loxP
    neo neomycin resistance gene (E. coli)13 mouse phosphoglycerate kinase promoter (PGK promoter)
    nls SV40 large T antigen nuclear localization signal (NLS)13nls
    puro puromycin acyltransferase (Streptomyces alboniger)13puro CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
    SV40 polyA13

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • increased rhabdomyosarcoma incidence(MP:0002036)

  • increased tumor incidence(MP:0002020)

  • myositis(MP:0004510)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, yeast HIS3 DNA, Streptomyces alboniger puromycin resistance gene, STOP sequence from pBS302, human K-rasG12V cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 nuclear localizing signal, E. coli lacZ, bovine Growth Hormone (GH) polyA signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 poly A aditional signal, mouse Ryr2 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorNoboru Suzuki (Mie University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data