Strain Data Sheet

RBRC09483

Strain Information

Image
BRC No.RBRC09483
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Rnf31<tm1.1Kiwa>
Former Common nameConditional HOIP delta linear mice, B6.B6CB-Hoip<tm1.1Kiwa>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Kazuhiro Iwai, Graduate School of Medicine, Kyoto University. TT2 ES cell line [(C57BL/6 x CBA)F1] was used. A pair of loxP sites were flanking exons encoding a RING-IBR-RING domain. A FRT-flanked Neo resistance gene was deleted. C57BL/6 congenic strain.
Strain descriptionFloxed mice of the Rnf31 (ring finger protein 31, HOIP) gene. Null mutants at whole body are lethal at embryonic day 10.5.
Colony maintenance
References
Defective immune responses in mice lacking LUBAC-mediated linear ubiquitination in B cells.
Sasaki Y, Sano S, Nakahara M, Murata S, Kometani K, Aiba Y, Sakamoto S, Watanabe Y, Tanaka K, Kurosaki T, Iwai K
EMBO J, 32, 2463-2476 (2013). 23942237

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site14Frt
Rnf31
MGI:1934704		ring finger protein 3114Rnf31<tm1.1Kiwa>
MGI:5550368		targeted mutation 1.1, Kazuhiro Iwai
loxP phage P1 loxP14loxP
loxP phage P1 loxP14loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal B-1 B cell morphology(MP:0004940)

  • abnormal humoral immune response(MP:0001800)

  • decreased B-1a cell number(MP:0008168)

  • decreased B-1b cell number(MP:0008170)

  • decreased IgA level(MP:0001807)
    • more 6 phenotypes
  • decreased IgG1 level(MP:0008495)

  • decreased IgG2a level(MP:0008496)

  • decreased IgG2b level(MP:0008497)

  • decreased IgG3 level(MP:0008498)

  • decreased IgM level(MP:0001806)

  • immune system phenotype(MP:0005387)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Rnf31 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J. 32, 2463-2476 (2013). The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKazuhiro Iwai (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Feb 2016

    BRC mice in Publications

    Fu Y, Li L, Zhang X, Deng Z, Wu Y, Chen W, Liu Y, He S, Wang J, Xie Y, Tu Z, Lyu Y, Wei Y, Wang S, Cui CP, Liu CH, Zhang L.
    Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.
    Nat Commun 15(1) 2974(2024) 38582895