Strain Data Sheet

RBRC09484

Strain Information

Image
BRC No.RBRC09484
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tmem119<tm2Tfur>
Former Common nameB6-Obif-KO, B6-Obif<tm2Tfur>
H-2 Haplotype
ES Cell lineJM8A3 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2015.
Strain descriptionObif, osteoblast induction factor gene knockout mice. Exon 2 of Obif gene was replaced with PGK-neo cassette.
Colony maintenance
References
OBIF, an osteoblast induction factor, plays an essential role in bone formation in association with osteoblastogenesis.
Mizuhashi K, Kanamoto T, Ito M, Moriishi T, Muranishi Y, Omori Y, Terada K, Komori T, Furukawa T
Dev. Growth Differ., 54(4):474-480 (2012). 22416756

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tmem119
MGI:2385228		transmembrane protein 1195Tmem119<tm2Tfur> targeted mutation 2, Takahisa Furukawa
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal5

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal bone trabecula morphology(MP:0010867)

  • abnormal circulating osteocalcin level(MP:0011922)

  • abnormal intramembranous bone ossification(MP:0008273)

  • abnormal seminiferous tubule size(MP:0006307)

  • abnormal sperm number(MP:0002673)
    • more 15 phenotypes
  • abnormal spermatid morphology(MP:0006380)

  • abnormal trabecular bone thickness(MP:0009345)

  • arrest of spermatogenesis(MP:0001155)

  • decreased bone mineralization(MP:0020137)

  • decreased bone trabecula number(MP:0010869)

  • decreased bone volume(MP:0010876)

  • decreased epididymis weight(MP:0004929)

  • decreased litter size(MP:0001935)

  • decreased testis weight(MP:0004852)

  • increased osteoid volume(MP:0010926)

  • short femur(MP:0003109)

  • short humerus(MP:0004351)

  • short radius(MP:0004355)

  • short tibia(MP:0002764)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 Poly A signal, mouse Tmem119 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
    DepositorTakahisa Furukawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data