Strain Information | |
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Image | |
BRC No. | RBRC09485 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.Cg-Xcr1<tm2(HBEGF/Venus)Ksho> |
Former Common name | XCR1-DTRvenus |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008. |
Strain description | A chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-DTRvenus mice were generated by knocking the gene encoding a fusion protein consisting of human diphteria toxin receptor (DTR or HBEGF) and a fluorescence protein, venus (derived from Dr. Atsushi Miyawaki at BRI) into the murine XCR1 gene locus. In XCR1-DTRvenus mice, XCR1-expressing cells can be detected by venus expression and ablated transiently by the administration of diphteria toxin. |
Colony maintenance | |
References | Critical roles of a dendritic cell subset expressing a chemokine receptor, XCR1. Yamazaki C, Sugiyama M, Ohta T, Hemmi H, Hamada E, Sasaki I, Fukuda Y, Yano T, Nobuoka M, Hirashima T, Iizuka A, Sato K, Tanaka T, Hoshino K, Kaisho T J. Immunol., 190, 6071-6082 (2013). 23670193 |
Health Report | |
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Examination Date / Room / Rack | 2024/11/18Room:4-ARack:GSentinel mouse program 2024/08/19Room:4-ARack:GSentinel mouse program 2024/05/20Room:4-ARack:GSentinel mouse program 2024/02/19Room:4-ARack:GSentinel mouse program 2023/11/20Room:4-ARack:GSentinel mouse program 2023/08/21Room:4-ARack:GSentinel mouse program 2023/05/22Room:4-ARack:GSentinel mouse program 2023/03/22Room:4-ARack:GAdditional tests |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 9 | Frt | ||||
Frt | yeast FRT (flippase recombination target) site | 9 | Frt | ||||
GH | Growth hormone polyA (Bovine) | 9 | GH | ||||
HBEGF | heparin-binding EGF-like growth factor (human) | 9 | HBEGF | ||||
Venus | Yellow Fluorescent Protein (Aequorea victoria) | 9 | Venus | ||||
Xcr1 MGI:1346338 | chemokine (C motif) receptor 1 | 9 | Xcr1<tm2(HBEGF/Venus)Ksho> MGI:5544058 | targeted mutation 2, Tsuneyasu Kaisho | |||
neo | neomycin resistance gene (E. coli) | 9 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 1 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | human HB-EGF (DTR) cDNA, Aequorea coerulescens mutant GFP (venus) cDNA, Bos taurus bGH polyA, Saccharomyces cerevisiae FRT sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Escherichia coli Neomycin resistance gene, HSV TK polyA signal, mouse Xcr1 genomic DNA |
Research application | FLP/frt system Fluorescent Proteins/lacZ System Immunology and Inflammation Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 190, 6071-6082 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Tsuneyasu Kaisho (RIKEN) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jan 2016 |
BRC mice in Publications |
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de Mingo Pulido Á, Hänggi K, Celias DP, Gardner A, Li J, Batista-Bittencourt B, Mohamed E, Trillo-Tinoco J, Osunmakinde O, Peña R, Onimus A, Kaisho T, Kaufmann J, McEachern K, Soliman H, Luca VC, Rodriguez PC, Yu X, Ruffell B. The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake. Immunity 54(6) 1154-1167.e7(2021) 33979578 |